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Transretinal biopsy technique for cytogenetic phenotyping of posterior uveal melanoma

DOI: 10.5677/2012-1-14

Submitted by: Javier Elizalde, MD, PhD

Cytogenetic phenotyping is an internationally-accepted procedure in ocular oncology to determine the metastatic likelihood of uveal melanoma. We describe our effective and safe vitreoretinal surgical technique to obtain a sample of melanomas located posterior to the equator. A three-port 25-gauge microcannula system through the pars plana is used to insert a vitrector or a 27-gauge needle to obtain the tissue, depending on the tumor size. Although minimal bleeding may occur during the immediate postoperative period, we observed no significant intraoperative or short-term complications in conjunction with this procedure.

Keywords: intraocular biopsy, transretinal biopsy, uveal melanoma, cytogenetics, vitrector-assisted biopsy

References:
1) Char DH, Kemlitz AE, Miller T. Intraocular biopsy. Ophthalmol Clin North Am. 2005;18(1):177-85
2) Midena E, Segato T, Piermarocchi S, Boccato P. Fine needle aspiration biopsy in ophthalmology. Surv Ophthalmol. 1985;29(6):410-22
3) Shields JA, Shields CL, Ehya H, Eagle RC Jr, De Potter P. Fine-needle aspiration biopsy of suspected intraocular tumors. The 1992 Urwick Lecture. Ophthalmology. 1993;100(11):1677-84
4) Sen J, Groenewald C, Hiscott PS, Smith PA, Damato BE. Transretinal choroidal tumor biopsy with a 25-gauge vitrector. Ophthalmology. 2006;113(6):1028-31
5) Shields JA, Shields CL, Materin M, Sato T, Ganguly A. Role of cytogenetics in management of uveal melanoma. Arch Ophthalmol. 2008;126(3):416-9

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Surgery of proliferative vitreoretinopathy (PVR): A step-by-step approach

Manish Nagpal, MS, FRCS, Vikram Mehta, MS

DOI: 10.5677/2012-1-12
Institution: Retina Foundation, Ahmedabad, India
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DOI: 10.5677/2012-1-13
Institution: Aurelios Eye Center, Recklinghausen, Germany
original video

Transretinal biopsy technique for cytogenetic phenotyping of posterior uveal melanoma

Javier Elizalde, MD, PhD

DOI: 10.5677/2012-1-14
Institution: Barraquer Ophthalmology Centre, Barcelona, Spain
ISSN: 2191-8473